In this experiment, we selected mice to establish a research model, isolated, purified and amplified the mouse GVHD model from the immunomagnetic bead separation of mouse bone marrow mesenchymal stem cells, and treated the mesenchymal stem cells with IFN-γ to infuse into mice, and further observed its effect on the inhibition of GVHD in mice. This evidence concerns the gene IFNG and graft versus host disease.