Given the importance of cell death in myocardial ischemia/reperfusion injury, to assess the effect of gAd on hypoxia/reoxygenation-induced cell death and further define the types of cell death, we costained the cells using a TUNEL assay and anti-cleaved caspase-3 antibodies, which allowed differentiating necroptotic cells (cleaved caspase-3−TUNEL+) from apoptotic cells (cleaved caspase-3+TUNEL+). Here, CASP3 is linked to myocardial ischemia.