So we choose MMP13 to evaluate degrading enzyme activity, and the results were identified that LY294002 upregulated MMP13 expression and IGF-1 downregulated MMP13 expression which consistent with the findings of Lu et al. that in the osteoarthritis rat model via activating P13K/AKT, MMP13 secretion was suppressed [32]. Here, IGF1 is linked to osteoarthritis.