To this end, we decided to compare four mycobacterial strains: two Mtb species—namely, the Mtb H37Rv reference strain for human TB and the cattle derived Mtb BTB1558—and two Mb species—namely, Mb AF2122 as representative of the EU1 clonal complex and Mb3601 as the hallmark EU3 strain. The gene discussed is MB; the disease is tuberculosis.