In addition, GSEA results suggest that some tumor-related pathways, such as P53 signaling pathway, DNA replication, cell cycle, and base excision repair, are enriched in the C1 subtype, while fatty acid metabolism, primary bile acid biosynthesis, renin, angiotensin, methionine and tyrosine metabolism are enriched in the C2 subtype, indicating that the poor prognosis of C1 subtype is related to the activation of tumor-related pathways and the inhibition of normal metabolism. The gene discussed is REN; the disease is neoplasm.