In our study, the co-culture system of BMSCs HS-5 and acute leukemia cells, including direct co-culture and indirect transwell co-culture to mimic the BMM was established, and the Annexin V-FITC analysis showed that HS-5 obviously suppressed the apoptosis of AML cells induced by Ara-c. The gene discussed is ANXA5; the disease is acute myeloid leukemia.