Moreover, we detected the expression of PAK1 and phosphorylated PAK1 at Ser144 (p-PAK1) in seven leukemia cell lines (THP1, Kasumi-1, U937, HL60, and KG1 are AML cell lines, K562 is a CML cell line, and Jurkat is a T-ALL cell line) by western blot analysis. This evidence concerns the gene PAK1 and acute lymphoblastic leukemia.