After PAK1 suppression by lentivirus with shRNA, THP1, and Kasumi-1 cells were treated with different concentrations of Ara-C (THP1: 0, 2, and 4 μM; Kasumi-1: 0, 4, and 8 μM) or IDA (THP1: 0, 10, and 20 μg/L; Kasumi-1: 0, 20, and 40 μg/L) for 24 h, and flow cytometry was performed to determine the apoptosis of AML cells induced by chemotherapy drugs. The gene discussed is PAK1; the disease is acute myeloid leukemia.