Nestin, as another putative marker for the GSC phenotype, probably plays a significant role in aggressive growth metastasis and self-renewal capacity of CSCs, organizing the cytoskeleton, cell signaling, organogenesis, and cell metabolism, and represents the proliferation, migration, and multi-differentiated characteristics of multi-lineage progenitor cells, and it is thus a more suitable target molecule to identify GSCs in GBM than CD133 (86, 87). The gene discussed is NES; the disease is glioblastoma.