CD4 and neoplasm: Because of the loss of mismatch repair protein gene (17), tumor cells showed relative poor differentiation and relatively more mucinous tissue and necrotic components exist (18), more CD3+, CD4+, CD8+, CD20+, and CD68+ cell infiltration and showed higher microvessel density (MVD) (19), resulting in different image grayscale distribution, which caused differences in image histogram features and texture features.