Based on this background, we hypothesized that estrogen might exert anti-tumorigenic effects and cooperate with Nrf2 deficiency in the immune microenvironment Therefore, this study aimed to determine the sex difference in PD-L1 expression and its correlation with Nrf2 expression in the colon tumor microenvironment of the AOM/DSS-induced CRC mouse model. This evidence concerns the gene NFE2L2 and colonic neoplasm.