To verify whether the regulation of MTA1 expression by FOXP3 affects the ability of breast cancer cells to metastasize in vitro, MDA-MB-231 cells with FOXP3 overexpression alone or in combination with MTA1 overexpression were generated, and changes in the cell invasion and migration abilities were observed through Transwell and wound healing assays. This evidence concerns the gene MTA1 and breast cancer.