On average, the CSF of controls contained 3.92 ± 0.23 ng/ml irisin and 912.8 ± 26.7 pg/ml klotho, the CSF of stroke patients at acute stage contained 2.12 ± 0.25 ng/ml irisin and 590.5 ± 36.3 pg/ml klotho, and the CSF of patients after recovery from stroke contained 2.83 ± 0.29 ng/ml irisin and 766.4 ± 43.8 pg/ml klotho. The gene discussed is FNDC5; the disease is Stroke.