In this study, autophagy protein microtubule-associated protein 1A/1B-Light chain 3 (LC3), p62/SQSTM1, and Beclin 1 were detected in ischemic stroke, and the key role that autophagy plays in ischemic stroke after NBO treatment was demonstrated by using NBO together with an intracerebroventricular injection of 3-MA. This evidence concerns the gene MAP1LC3A and ischemic stroke.