Using the GSCALite to analyze the cancer potential pathways of HOXBs, we found that HOXB9 promotes DNA damage response, HOXB9/13 promote cell apoptosis, HOXB6/7 suppress PI3K/AKT classic cancer pathway, HOXB4/5/6/7/9 suppress receptor tyrosine kinase (RTK) pathway, HOXB3/5 promote hormone androgen receptor (AR), HOXB3/4/5/9 suppress hormone estrogen receptor (ER), HOXB2/6 suppress cell cycle, HOXB3/6/7/9 suppress hormone ER, and HOXB7 suppresses RTK pathway. This evidence concerns the gene HOXB4 and cancer.