GNAI1 and Anxiety: To test the hypothesis that VMH astrocyte activity mediates chronic stress-induced anxiety-like behavior and decreases the BMD, we injected AAV2-DIO-hM4Di-mCherry or AAV2-DIO-mCherry into the VMH of S100β-Cre mice, which enabled the selective activation of the Gi pathway in VMH astrocytes by clozapine N-oxide (CNO) administration (Figures 3(a) and 3(b)).