We first assessed the expression and prognostic significance of CD161 in pan-cancer and found that its expression was high in 14 tumours, including ACC, CESC, ESCA, GBM, KIRC, KIRP, LAML, LGG, OV, PAAD, READ, SKCM, STAD, and TGCT. This evidence concerns the gene KLRB1 and neoplasm.