Moreover, we took advantage of the stable Miz1 knockout (KO) cells available in our laboratory, which was established using Crispr/Cas 9 system in mouse lung adenocarcinoma cells originated from tumors of Cre recombinase-treated KrasLSL-G12D/+Trp53fl/f mice, which carries a lox-stop-lox (LSL) sequence followed by the KRAS G12D point mutation allele as well as loxP sites flanking exons 2–10 of the transformation related protein 53 (Trp53) gene as reported (referred to hereafter as KP cells) (21, 22). The gene discussed is TP53; the disease is lung adenocarcinoma.