In the TCGA-NSCLC cohort, the proportions of various activated immune cells, such as M0 macrophages, M1 macrophages, activated memory CD4+ T cells and CD8+ T cells, in the high-mutated NOTCH group were significantly higher than those in the low-mutated NOTCH group (all P < 0.05; Figure 5A). This evidence concerns the gene CD8A and non-small cell lung carcinoma.