NOTCH1 and ischemic stroke: In addition, qPCR (Figures 4D,E) and western blot (Figure 3D) revealed that after ischemic stroke, the expression of Notch1, Jagged1, and Hes1 mRNA (Figure 4D) and Notch1, Jagged1, NICD, and HES1 protein (Figures 3D, 4F) was significantly increased at D1 and D3 in NDI group.