We observed that GSDME has low expression in MDA-MB-231 breast cancer cells; at the same time, we also noticed that the ratio of lytic death (annexin V+/PI+) was lower in MDA-MB-231 cells compared to MCF-7 cells, but we were unclear whether the low expression level of GSDME due to GSDME methylation leads to the lower ratio of lytic death. This evidence concerns the gene GSDME and breast carcinoma.