To further investigate the underlying mechanisms of triclabendazole-induced apoptosis and lytic cell death in breast cancer cells, we next analyzed the protein markers of apoptosis by immunoblotting, including Bax, Bcl-2, cleaved caspase-9, cleaved caspase-8, cleaved caspase-3, cleaved caspase-7, and cleaved PARP. This evidence concerns the gene CASP9 and breast carcinoma.