We observed that GSDME has low expression in MDA-MB-231 breast cancer cells; at the same time, we also noticed that the ratio of lytic death (annexin V+/PI+) was lower in MDA-MB-231 cells compared to MCF-7 cells, but we were unclear whether the low expression level of GSDME due to GSDME methylation leads to the lower ratio of lytic death. The gene discussed is GSDME; the disease is breast cancer.