Hence, the aim of the present study was to investigate the specific mechanism underlying the role of APPL1 in myocardial injury caused by ischemia/reperfusion and clarified whether caspase9 /APAF-1 is involved in the mechanism of APPL1 in myocardial ischemia/reperfusion model in vivo/vitro. Here, APPL1 is linked to myocardial ischemia.