Furthermore, we confirmed that 6 of the 8 osteosarcoma cell lines, including MG-63, HuO9, HuO-3N1, G-292, NY, and SJSA-1, expressed high LPAR1 levels by qPCR and immunoblotting analysis (Fig. 2D, E). The gene discussed is LPAR1; the disease is osteosarcoma.