SMYD3 and hepatocellular carcinoma: We assessed the effects of altered expression, including SMYD3 knockdown, SMYD3 overexpression, S1PR1 overexpression, and the combination of SMYD3 knockdown and S1PR1 overexpression, on the growth of local tumors in nude mice to test whether SMYD3 promotes HCC progression via S1PR1 in vivo (Fig. 6E).