Overall, these results indicate that MYO1F plays a critical role in innate antifungal immunity by regulating Syk/CARD9 “membrane to cytoplasm trafficking,” that the deacetylase Sirt2 is a good therapeutic target for antifungal drug development, and that inhibitors of Sirt2 may be developed as potential drugs for the treatment of fungal infection. Here, SIRT2 is linked to fungal infectious disease.