We found that the concentrations of TGF-β, IL-10, and IL-21 were decreased while the levels of GM-CSF, IL-17A, IL-17F, IL-22, and IL-23 were increased in constipation EAE mice and FMT EAE mice compared to EAE mice (Fig. 6). This evidence concerns the gene TGFB1 and constipation disorder.