CASP3 and intrahepatic cholangiocarcinoma: Additionally, the results of ICC for caspase-3 and MTT in 3D were very similar, as both experiments showed that Fx and Dox 1 μM alone did not present differences in relation to the control, while Dox ≥ 1 μM in combination with Fx revealed differences that show cell cytotoxicity and apoptosis induction.