Subsequently, it was found that NlFAS2, NlFAD3, NlELO2 and NlELO16 were significantly up-regulated under both low and high temperatures in BPH, and the up-regulated genes in WBPH were related to ACC, FAS and FAD, but in SBPH they were just related to LsELO18 under 38 °C. The gene discussed is FAS; the disease is benign prostatic hyperplasia.