To address eventual association between CD73 positivity and (loss of) podocyte-specific protein expression, we performed additional immunofluorescent stainings for nephrin and podocin and evaluated their expression pattern in control, MCD and FSGS groups (Figure 6a) as well as co-staining with CD73 in samples with podocyte lesion (Figure 6b–e). Here, NPHS2 is linked to focal segmental glomerulosclerosis.