In this study, we have identified a pseudogene-derived lncRNA (PLEKHA8P1) and its corresponding parental gene (PLEKHA8), which promotes chemoresistance to 5-fluorouracil (5-FU) in the human HCC cell line FT3-7 (clonal derivative of Huh-7 cells). Here, PLEKHA8P1 is linked to hepatocellular carcinoma.