Nonetheless, our study revealed the novel role of PLEKHA8P1 in HCC cell line as an oncogenic pseudogene-lncRNA and confirmed cancer-related functions of its parental gene PLEKHA8. Our data suggest that PLEKHA8P1 positively regulates PLEKHA8 as a miRNA sponge, and alteration of PLEKHA8P1 expression affects chemosensitivity of HCC cell line FT3-7 to 5-FU, highlighting the potential of PLEKHA8P1 as a novel therapeutic target in chemoresistance in liver cancer. The gene discussed is PLEKHA8; the disease is cancer.