The UHN Melanoma NGS Panel examined exonic coding regions of BAP1, BRAF, CDK4, CDK6, CDKN2A, GNA11, GNAQ, KIT, NRAS, EIF1AX, and SF3B1. This methodology employs SureSelect Target Enrichment hybrid capture followed by paired-end sequencing on the Illumina sequencing platform. This evidence concerns the gene EIF1AX and melanoma.