AIF1 and glioblastoma: Confocal analysis of immunofluorescence and transgenic markers (Figure 4B) revealed that the majority of Iba1-positive TAM in primary GBM were RFP-positive microglia (a facilitated view on all colocalizing pixels for the confocal channels recording Iba1 and RFP is given in the micrographs termed “Colocalization”; Figure 4B).