With this strategy we showed, for the first time, that the CP-CML clone at diagnosis has a characteristic DNA methylation profile with an overall tendency to hypomethylation (33 CML-specific abnormally methylated hotspots), and also that the immature CD34+ CD15- subpopulation has a specific profile with a more pronounced hypomethylation. Here, CD34 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.