Using the Cancer Cell Line Encyclopedia (CCLE) RNA-seq data of human prostate cancer cell lines, 22Rv1, DU145, LNCaP, MDA PCa 2b, NCI-H660, PC-3, and VCaP, we observed that, compared with SHBG, which is endogenously not expressed, SRD5A1 is significantly upregulated in recurrent and metastatic PCa cell lines (Figure 5A). Here, SHBG is linked to Familial prostate cancer.