To validate the dependency of tumor cells on CSNK1A1, KDM2A, and LTB4R2 in vitro, we generated the knockout (KO) of each gene by independently introducing two sgRNAs in two AD cell lines, NCI-H23 and A549 (Figure 6A,C and Supplementary Figure S1). This evidence concerns the gene KDM2A and Alzheimer disease.