To validate the dependency of tumor cells on CSNK1A1, KDM2A, and LTB4R2 in vitro, we generated the knockout (KO) of each gene by independently introducing two sgRNAs in two AD cell lines, NCI-H23 and A549 (Figure 6A,C and Supplementary Figure S1). The gene discussed is CSNK1A1; the disease is Alzheimer disease.