Briefly, by mutagenesis of the NPM1 wild type plasmid [21] we generated vectors expressing NPM1 mutation type A, B and NPM1 mutation D with the loss of both Trp residues (A-like mutations) and vectors expressing NPM1 mutation 7 and 8, where only one Trp residue is lost (non-A-like mutations) derived from de novo NPM1-mutated AML samples. Here, NPM1 is linked to acute myeloid leukemia.