HOXB5 and acute myeloid leukemia: To obtain deeper insights into the correlation between HOX family gene expression and NPM1 mutational status, we transfected HL-60 with the pEGFP-NPM1wt, pEGFP-NPM1mutB, and pEGFP-NPM1mut7 plasmids and analyzed HOXA5, HOXA10, and HOXB5 expression in EGFP+-sorted cells, previously described as the most aberrantly expressed HOX genes in NPM1-mutated AML [31].