The reason for the contradictory results from different laboratories with the same cell line is not clear except Santi et al. [71] and Bai et al. [107] depleted Akt2 by siRNA or miRNA and monitored overall cell growth using MTT assay whereas Toulany et al. selected shRNA-transfected MDA-MB-231 cells and assessed clonogenic activity in vitro and tumor growth in vivo [82]. Here, AKT2 is linked to neoplasm.