Consistent with previous observations [22,25,32], metformin not only effectively suppressed nuclear YAP activity by means of increased YAP phosphorylation in APC-mutated CRC cells, but also moderately suppressed canonical Wnt activity, the Snail-mediated EMT program, and mTOR activity (Figure S3), indicating that metformin can attenuate niclosamide-mediated oncogenic YAP activation as well as suppress Wnt activity. This evidence concerns the gene MTOR and colorectal carcinoma.