It has been reported that unfractionated self-peripheral blood mononuclear cells (PBMC) can be co-cultured for a long time with epithelial cell organoids from CRC or non-small cell lung carcinoma (NSCLC) in the presence of IL2, IFNγ, anti-CD3 and anti-CD28 antibodies coated to plastic for 28 days. This evidence concerns the gene CD28 and non-small cell lung carcinoma.