PKM and urinary bladder cancer: Figure 5A shows that siPKM2 transfection effectively silenced PKM2 protein expression, even when DPYSL2 was overexpressed. Compared with negative control, PKM2 silencing not only substantially inhibited bladder cancer cell proliferation, clone formation, migration, and invasion but also completely abolished DPYSL2-induced enhancement of these malignant behaviors (Figures 5B–D).