We confirmed the downregulation of Alkbh5 and Fto in infected IEC4.1 cells at the RNA level (using real-time PCR, Figure 2B) and at the protein level (using Western blot, Figure 2C), as well as at the RNA level in isolated intestinal epithelium from neonatal mice of intestinal cryptosporidiosis through oral administration of the parasite (23, 24) (Figure 2D), and infected 2D enteroid monolayers from neonatal mouse ileum (Figure 2E). This evidence concerns the gene FTO and cryptosporidiosis.