Eight PRRs were identified as up-regulated after the second immune challenge, including perlucin 4, C1q domain containing protein 2, scavenger receptor class B-like protein, deleted in malignant brain tumors 1 protein-like, TLR, TLR2, TLR3 and TLR8 (Figure 4B). The gene discussed is TLR8; the disease is brain cancer.