Infection of host cells by L. monocytogenes can be divided into three stages that require specific virulence factors: Initial cell invasion (InlA and InlB), escape from vacuole (Hly, PlcA, and PlcB) and cell-to-cell spread (ActA and UhpT) (Cossart et al., 1989; Mélanie et al., 2006; Schnupf and Portnoy, 2007; Joyce and Gahan, 2010; Hamon et al., 2012). Here, ACTA1 is linked to infection.