PSMA is predominantly expressed in the prostate gland and its expression has been shown to be elevated in PC and correlated with various clinicopathological features of PC patients13,16,31.We isolated PSMA-EVs from serum by immunocapture using anti-PSMA antibody and detected them on Western blot using anti-CD9 antibody, demonstrating their presence in serum25. This evidence concerns the gene FOLH1 and pachyonychia congenita.