CTLA4-cg22572158 is of particular interest because it is known to be associated with diabetes risk31 and it is worth noting that this particular CpG was over-methylated at baseline as compared to Non-Diabetic Controls (Fig. 5c) and then gradually demethylated during BCG therapy, with the largest demethylation in year 3 (Fig. 5d). Here, CTLA4 is linked to diabetes mellitus.