To screen candidate drugs for clinical use in ESCC and lymph node metastasis, we performed an integrated analysis of our tumor-specific SE-linked genes with the Washington University Drug Gene interaction database42, informing 69 common gained SE-linked genes and 43 LNC-specific gained SE-linked genes, which were redundantly categorized into 21 and 18 classes, respectively, including druggable genome, kinase, clinically actionable, cell surface, TF binding, etc. (Fig. 5a, b and Supplementary Data 21). Here, TF is linked to metastatic malignant neoplasm in the lymph nodes.