A negative prognostic effect of gene mutation has been confirmed in a large pooled analysis from the PETACC8 and N0147 trials, where KRAS exon 2 and BRAF mutations were identified as independent predictors of shorter time to recurrence and overall survival (OS) among patients with stage III microsatellite stable (MSS) colorectal cancer [26]. This evidence concerns the gene BRAF and colorectal cancer.