As demonstrated by flow cytometric analysis, EC cell lines 2102EP, NCCIT, and NT2/D1 (NANOG+/OCT3/4+/SOX2+) presented as CD24+/CD44+/CD133+/CXCR4‐, seminoma‐like TCam‐2 (NANOG+/OCT3/4+/SOX17+) as CD24‐/CD44‐/CD133+/CXCR4+, YST cell lines 1411H and GCT72 (NANOG‐/OCT3/4‐/SOX2‐) as CD24‐/CD44‐/CD133‐/CXCR4+, and the CC cell lines JAR, JEG3, and BeWo (NANOG‐/OCT3/4‐/SOX2‐) as CD24‐/CD44‐/CD133‐/CXCR4‐ (Figs 1A and 2A). The gene discussed is PROM1; the disease is cholangiocarcinoma.