Clemastine can pass through the blood brain barrier and improved myelination and neuronal function in a clinical trial of multiple sclerosis [59]. It is not completely clear in this case that clemastine enhances myelination solely by blocking muscarinic receptor 1 (M1R), given its binding affinity to a wide range of receptors, such as histamine receptors and other muscarinic receptor subtypes [60], and it was not clear that clemastine could regulate oxygen level or Olig2 expression directly. Here, OLIG2 is linked to multiple sclerosis.