Tracking the expression of this NF-κB gene signature across infection status (Fig. 3 f) reveals a pattern similar to Zeb2 and Cxcl2, suggesting that upon infection with Mtb, a subpopulation of AM_2 cells segregates within the IM_1 cluster, while others mount a pro-inflammatory response that is similar to IM_3. This evidence concerns the gene NFKB1 and infection.