Altogether we have established a novel syngeneic OSCC grafting model derived from a 4NQO-induced tongue tumor that showed similarities with human OSCC by recapitulating mutations seen in human tumors, constitutive TGFβ signaling, abundant TIL within TNC-rich stroma and, spontaneous tumor cell dissemination to and expansion in the local lymph nodes. Here, TGFB1 is linked to tongue neoplasm.