Among all tested individuals, a total of 28 MANA-specific CD8 clonotypes (1,350 total cells from 3 patients with MPR and 3 patients without MPR) as identified by MANAFEST were detected in the single-cell data, of which 20 clonotypes (890 cells) were in the tumour (Fig. 2c, Supplementary Table 8). This evidence concerns the gene CD8A and neoplasm.